June 23, 2022

Mesaconate is synthesized from itaconate and exerts immunomodulatory effects on macrophages

  • Michelucci, A. et al. Immunosensitive gene 1 protein links metabolism to immunity by catalyzing the production of itaconic acid. proc. Natl Acad. Science. UNITED STATES 1107820–7825 (2013).

    CASE
    Article

    Google Scholar

  • Mills, EL et al. Itaconate is an anti-inflammatory metabolite that activates Nrf2 via alkylation of KEAP1. Nature 556113-117 (2018).

    CASE
    Article

    Google Scholar

  • Bambouskova, M. et al. The electrophilic properties of itaconate and its derivatives regulate the inflammatory IκBζ–ATF3 axis. Nature 556501–504 (2018).

    CASE
    Article

    Google Scholar

  • Wynn, TA, Chawla, A. & Pollard, JW Biology of macrophages in development, homeostasis, and disease. Nature 496445–455 (2013).

    CASE
    Article

    Google Scholar

  • He, W., Heinz, A., Jahn, D. & Hiller, K. Complexity of macrophage metabolism in infection. Running. Notice. Biotechnol. 68231–239 (2021).

    CASE
    Article

    Google Scholar

  • Cordes, T. et al. Immunosensitive gene 1 and itaconate inhibit succinate dehydrogenase to modulate intracellular succinate levels. J. Biol. Chem. 29114274–14284 (2016).

    CASE
    Article

    Google Scholar

  • Nemeth, B. et al. Abolition of mitochondrial substrate level phosphorylation by itaconic acid produced by LPS-induced Irg1 expression in murine macrophage lineage cells. FASB J. 30286–300 (2016).

    CASE
    Article

    Google Scholar

  • Cordes, T. & Metallo, CM Itaconate modifies succinate and coenzyme a metabolism via inhibition of mitochondrial complex II and methylmalonyl-CoA mutase. Metabolites 11117 (2021).

  • Lampropoulou, V. et al. Itaconate links succinate dehydrogenase inhibition to metabolic remodeling of macrophages and regulation of inflammation. Metab cell. 24158-166 (2016).

    CASE
    Article

    Google Scholar

  • Liao, ST et al. 4-Octyl itaconate inhibits aerobic glycolysis by targeting GAPDH to exert anti-inflammatory effects. Nat. Common. ten5091 (2019).

    Article

    Google Scholar

  • Qin, W. et al. Cysteine ​​profiling based on S-glycosylation reveals the regulation of glycolysis by itaconate. Nat. Chem. Biol. 15983–991 (2019).

    CASE
    Article

    Google Scholar

  • ElAzzouny, M. et al. Dimethyl itaconate is not metabolized intracellularly to itaconate. J. Biol. Chem. 2924766–4769 (2017).

    CASE
    Article

    Google Scholar

  • Swain, A. et al. Comparative evaluation of itaconate and its derivatives reveals divergent regulation of inflammasome and type I interferon in macrophages. Nat. Metab. 2594–602 (2020).

    CASE
    Article

    Google Scholar

  • Bambouskova, M. et al. Itaconate confers tolerance to late NLRP3 inflammasome activation. Cell representative 34108756 (2021).

    CASE
    Article

    Google Scholar

  • Ghosn, EE et al. Two physically, functionally and developmentally distinct subsets of peritoneal macrophages. proc. Natl Acad. Science. UNITED STATES 1072568-2573 (2010).

    CASE
    Article

    Google Scholar

  • Wang, J. & Zhang, K. Production of mesaconate in Escherichia coli via the modified glutamate mutase pathway. Metab. Eng. 30190-196 (2015).

    Article

    Google Scholar

  • Meiser, J. et al. Pro-inflammatory macrophages support pyruvate oxidation by pyruvate dehydrogenase for itaconate synthesis and to enable cytokine expression. J. Biol. Chem. 2913932–3946 (2016).

    CASE
    Article

    Google Scholar

  • Hooftman, A. et al. The immunomodulatory metabolite itaconate modifies NLRP3 and inhibits inflammasome activation. Metab cell. 32468–478 (2020).

    CASE
    Article

    Google Scholar

  • Broz, P. & Dixit, VM Inflammasomes: assembly, regulation and signaling mechanism. Nat. Rev. Immunol. 16407–420 (2016).

    CASE
    Article

    Google Scholar

  • Mangan, MSJ et al. Targeting the NLRP3 inflammasome in inflammatory diseases. Nat. Rev. Drug Disco. 17688 (2018).

    CASE
    Article

    Google Scholar

  • Chen, F et al. Citraconate inhibits ACOD1 (IRG1) catalysis, reduces interferon responses and oxidative stress, and modulates inflammation and cellular metabolism. Nat. Metab.

  • Kornberg, MD et al. Dimethyl fumarate targets GAPDH and aerobic glycolysis to modulate immunity. Science 360449-453 (2018).

    CASE
    Article

    Google Scholar

  • Montes Diaz, G., Hupperts, R., Fraussen, J. & Somers, V. Treatment of dimethyl fumarate in multiple sclerosis: recent advances in clinical and immunological studies. Autoimmune. Round. 171240-1250 (2018).

    CASE
    Article

    Google Scholar

  • Hartman, MG et al. Role of transcription factor 3 activation in stress-induced beta-cell apoptosis. Mol. Cell. Biol. 245721–5732 (2004).

    CASE
    Article

    Google Scholar

  • Chen, Y. et al. Specific to hepatocytes CGLC suppression leads to the rapid onset of steatosis with mitochondrial damage and liver failure. Hepatology 451118-1128 (2007).

    CASE
    Article

    Google Scholar

  • Clausen, BE, Burkhardt, C., Reith, W., Renkawitz, R. & Forster, I. Conditional gene targeting in macrophages and granulocytes using LysMcre mice. Transgenic Res. 8265-277 (1999).

    CASE
    Article

    Google Scholar

  • Weindl, D. et al. Bridging the gap between off-target stable isotope labeling and metabolic flux analysis. Cancer Metab. 410 (2016).

    Article

    Google Scholar

  • Sapcariu, SC et al. Simultaneous extraction of proteins and metabolites from cultured cells. MethodsX 174–80 (2014).

    Article

    Google Scholar

  • He, W. et al. TLR4 triggered complex inflammation in human pancreatic islets. Biochem. Biophys. Acta Mol. Base Dis. 186586–97 (2019).

    CASE
    Article

    Google Scholar

  • Battello, N. et al. The role of HIF-1 in oncostatin M-dependent metabolic reprogramming of liver cells. Cancer Metab. 43 (2016).

    Article

    Google Scholar

  • Hiller, K. et al. MetaboliteDetector: comprehensive analysis tool for targeted and non-targeted analysis of the metabolome based on GC/MS. Anal. Chem. 813429–3439 (2009).

    CASE
    Article

    Google Scholar

  • Nonnenmacher, Y. et al. Analysis of mitochondrial metabolism in situ: combining stable isotopic labeling and selective permeabilization. Metab. Eng. 43147-155 (2017).

    CASE
    Article

    Google Scholar

  • Nonnenmacher, Y., Palorini, R. & Hiller, K. Determination of compartment-specific metabolic fluxes. Methods Mol. Biol. 1862137-149 (2019).

    CASE
    Article

    Google Scholar

  • Afgan, E. et al. The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2018 update. Nucleic Acids Res. 46W537–W544 (2018).

    CASE
    Article

    Google Scholar

  • Kim, D., Langmead, B. & Salzberg, SL HISAT: A fast spliced ​​aligner with low memory requirements. Nat. Methods 12357–360 (2015).

    CASE
    Article

    Google Scholar

  • Robinson, MD, McCarthy, DJ & Smyth, GK edgeR: A Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics 26139-140 (2010).

    CASE
    Article

    Google Scholar

  • Yu, G., Wang, LG, Han, Y. & He, QY clusterProfiler: an R package for comparing biological themes between gene clusters. OMIC 16284-287 (2012).

    CASE
    Article

    Google Scholar


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