Michelucci, A. et al. Immunosensitive gene 1 protein links metabolism to immunity by catalyzing the production of itaconic acid. proc. Natl Acad. Science. UNITED STATES 1107820–7825 (2013).
Google Scholar
Mills, EL et al. Itaconate is an anti-inflammatory metabolite that activates Nrf2 via alkylation of KEAP1. Nature 556113-117 (2018).
Google Scholar
Bambouskova, M. et al. The electrophilic properties of itaconate and its derivatives regulate the inflammatory IκBζ–ATF3 axis. Nature 556501–504 (2018).
Google Scholar
Wynn, TA, Chawla, A. & Pollard, JW Biology of macrophages in development, homeostasis, and disease. Nature 496445–455 (2013).
Google Scholar
He, W., Heinz, A., Jahn, D. & Hiller, K. Complexity of macrophage metabolism in infection. Running. Notice. Biotechnol. 68231–239 (2021).
Google Scholar
Cordes, T. et al. Immunosensitive gene 1 and itaconate inhibit succinate dehydrogenase to modulate intracellular succinate levels. J. Biol. Chem. 29114274–14284 (2016).
Google Scholar
Nemeth, B. et al. Abolition of mitochondrial substrate level phosphorylation by itaconic acid produced by LPS-induced Irg1 expression in murine macrophage lineage cells. FASB J. 30286–300 (2016).
Google Scholar
Cordes, T. & Metallo, CM Itaconate modifies succinate and coenzyme a metabolism via inhibition of mitochondrial complex II and methylmalonyl-CoA mutase. Metabolites 11117 (2021).
Lampropoulou, V. et al. Itaconate links succinate dehydrogenase inhibition to metabolic remodeling of macrophages and regulation of inflammation. Metab cell. 24158-166 (2016).
Google Scholar
Liao, ST et al. 4-Octyl itaconate inhibits aerobic glycolysis by targeting GAPDH to exert anti-inflammatory effects. Nat. Common. ten5091 (2019).
Google Scholar
Qin, W. et al. Cysteine profiling based on S-glycosylation reveals the regulation of glycolysis by itaconate. Nat. Chem. Biol. 15983–991 (2019).
Google Scholar
ElAzzouny, M. et al. Dimethyl itaconate is not metabolized intracellularly to itaconate. J. Biol. Chem. 2924766–4769 (2017).
Google Scholar
Swain, A. et al. Comparative evaluation of itaconate and its derivatives reveals divergent regulation of inflammasome and type I interferon in macrophages. Nat. Metab. 2594–602 (2020).
Google Scholar
Bambouskova, M. et al. Itaconate confers tolerance to late NLRP3 inflammasome activation. Cell representative 34108756 (2021).
Google Scholar
Ghosn, EE et al. Two physically, functionally and developmentally distinct subsets of peritoneal macrophages. proc. Natl Acad. Science. UNITED STATES 1072568-2573 (2010).
Google Scholar
Wang, J. & Zhang, K. Production of mesaconate in Escherichia coli via the modified glutamate mutase pathway. Metab. Eng. 30190-196 (2015).
Google Scholar
Meiser, J. et al. Pro-inflammatory macrophages support pyruvate oxidation by pyruvate dehydrogenase for itaconate synthesis and to enable cytokine expression. J. Biol. Chem. 2913932–3946 (2016).
Google Scholar
Hooftman, A. et al. The immunomodulatory metabolite itaconate modifies NLRP3 and inhibits inflammasome activation. Metab cell. 32468–478 (2020).
Google Scholar
Broz, P. & Dixit, VM Inflammasomes: assembly, regulation and signaling mechanism. Nat. Rev. Immunol. 16407–420 (2016).
Google Scholar
Mangan, MSJ et al. Targeting the NLRP3 inflammasome in inflammatory diseases. Nat. Rev. Drug Disco. 17688 (2018).
Google Scholar
Chen, F et al. Citraconate inhibits ACOD1 (IRG1) catalysis, reduces interferon responses and oxidative stress, and modulates inflammation and cellular metabolism. Nat. Metab.
Kornberg, MD et al. Dimethyl fumarate targets GAPDH and aerobic glycolysis to modulate immunity. Science 360449-453 (2018).
Google Scholar
Montes Diaz, G., Hupperts, R., Fraussen, J. & Somers, V. Treatment of dimethyl fumarate in multiple sclerosis: recent advances in clinical and immunological studies. Autoimmune. Round. 171240-1250 (2018).
Google Scholar
Hartman, MG et al. Role of transcription factor 3 activation in stress-induced beta-cell apoptosis. Mol. Cell. Biol. 245721–5732 (2004).
Google Scholar
Chen, Y. et al. Specific to hepatocytes CGLC suppression leads to the rapid onset of steatosis with mitochondrial damage and liver failure. Hepatology 451118-1128 (2007).
Google Scholar
Clausen, BE, Burkhardt, C., Reith, W., Renkawitz, R. & Forster, I. Conditional gene targeting in macrophages and granulocytes using LysMcre mice. Transgenic Res. 8265-277 (1999).
Google Scholar
Weindl, D. et al. Bridging the gap between off-target stable isotope labeling and metabolic flux analysis. Cancer Metab. 410 (2016).
Google Scholar
Sapcariu, SC et al. Simultaneous extraction of proteins and metabolites from cultured cells. MethodsX 174–80 (2014).
Google Scholar
He, W. et al. TLR4 triggered complex inflammation in human pancreatic islets. Biochem. Biophys. Acta Mol. Base Dis. 186586–97 (2019).
Google Scholar
Battello, N. et al. The role of HIF-1 in oncostatin M-dependent metabolic reprogramming of liver cells. Cancer Metab. 43 (2016).
Google Scholar
Hiller, K. et al. MetaboliteDetector: comprehensive analysis tool for targeted and non-targeted analysis of the metabolome based on GC/MS. Anal. Chem. 813429–3439 (2009).
Google Scholar
Nonnenmacher, Y. et al. Analysis of mitochondrial metabolism in situ: combining stable isotopic labeling and selective permeabilization. Metab. Eng. 43147-155 (2017).
Google Scholar
Nonnenmacher, Y., Palorini, R. & Hiller, K. Determination of compartment-specific metabolic fluxes. Methods Mol. Biol. 1862137-149 (2019).
Google Scholar
Afgan, E. et al. The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2018 update. Nucleic Acids Res. 46W537–W544 (2018).
Google Scholar
Kim, D., Langmead, B. & Salzberg, SL HISAT: A fast spliced aligner with low memory requirements. Nat. Methods 12357–360 (2015).
Google Scholar
Robinson, MD, McCarthy, DJ & Smyth, GK edgeR: A Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics 26139-140 (2010).
Google Scholar
Yu, G., Wang, LG, Han, Y. & He, QY clusterProfiler: an R package for comparing biological themes between gene clusters. OMIC 16284-287 (2012).
Google Scholar
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